8/26/10

(B; Noon)

Lesson Unit/Topic

• Introduction

• Syllabus

Target Gene research

8/30/10

(D)

Lesson Unit/Topic

• Conversions

• Discussion of target gene choice

9/1/10

(F; Summer Reading Test; First Friday Schedule)

Lesson Unit/Topic

• Pipetting refresher

• Safety precautions/equipment

• Problem solving with formula

.Review DNA

9/3/10

(B; First Friday)

Lesson Unit/Topic

• Pipetting practice

• DNA study

.Lab contracts signed

Review DNA

9/8/10

(D; Class Meetings)

Lesson Unit/Topic

• DNA study

.Diagrams and explanations for Transcription and Translation

9/10/10

(F)

Lesson Unit/Topic

• RNA and transcription

• Post transcriptional modification

• Post translational modification

.RNAi Worksheet in resources folder

9/14/10

(B)

Lesson Unit/Topic

• Protein Challenge

.Read Crick's original article on Central Dogma and be ready for class discussion in light of recent developments

9/16/10

(D)

Lesson Unit/Topic

• Final Discussion and review of protein challenge

.Study for test

9/21/10

(F)

Lesson Unit/Topic

• Test on nucleic acids

• presentation

.PCR worksheet; intranet

9/23/10

(B)

Lesson Unit/Topic

• PCR Overview and primer design

.Problem;  intranet

9/27/10

(D)

Lesson Unit/Topic

• review test and problems

• team analysis

• ncbi

.primer design

9/29/10

(F)

Lesson Unit/Topic

• primer analysis

.primer design

10/1/10

(B; First Friday)

Lesson Unit/Topic

• assessment of primer pairs

primer comparisons

10/5/10

(D)

Lesson Unit/Topic

• primers decisions

• DNA Extraction

Read protocol for extraction and be prepared.  Complete primer design with new info emailed to you. Use your own organism.

10/7/10

(F)

Lesson Unit/Topic

• DNA extraction

.Flow Chart and V Map

10/12/10

(B)

Lesson Unit/Topic

• Purification

.

10/14/10

(D)

Lesson Unit/Topic

• DNA extraction

Design PCR protocols

10/18/10

(F; 90-minute delay)

Lesson Unit/Topic

• Purification

.Design a flow chart from L. Pentz Protcol in Binder on DNA Extraction (calligraphy pages)

10/20/10

(B)

Lesson Unit/Topic

• primer math/reconstitute primers

• primer dilution

• procedural decisions

.study for quiz

extraction flow chart

10/22/10

(D; Advisee Meeting; Grades Close)

Lesson Unit/Topic

• Quiz: extraction/purification and primer design

• primer math

V map and flow chart #2.

10/26/10

(F)

Lesson Unit/Topic

•  PCR components

• Stock and working solns

Set up reactions in lab notebook

10/28/10

(B)

Lesson Unit/Topic

• PCR I

.PCR 1 flow chart

11/1/10

(D; All Saints Day Liturgy)

Lesson Unit/Topic

• PCR I and II

. V Map

11/3/10

(F; Noon; Report Cards)

Lesson Unit/Topic

• Run Gels and analyze

troubleshooting and decisions; see hand-in folder for pictures

11/5/10

(B; First Friday)

Lesson Unit/Topic

• PCR troubleshoot/corrections student decisions

• PCR set 3

.Set up table: lab notebook of results

Lab report: V map and flow chart due Nov. 12

11/9/10

(D)

Lesson Unit/Topic

• Run gels

• PCR 4

.

11/12/10

(F; Musical)

Lesson Unit/Topic

• Catch up

.Lab report with transformations

11/16/10

(B)

Lesson Unit/Topic

• run gels

• get test date

• take inventory

.

11/18/10

(D)

Lesson Unit/Topic

• Run gels

• take inventory

.Lab report due Monday by 10AM in smj's office on round table

11/22/10

(F)

Lesson Unit/Topic

• FTA purification

.

11/24/10

(B; Noon)

Lesson Unit/Topic

• No class

.

11/30/10

(D; Mid-Quarter Reports)

Lesson Unit/Topic

• PCR

• Purification introduction

.Study for Test

12/2/10

(F)

Lesson Unit/Topic

• PCR test

• Run gels

.Purification Flow Chart and data table

12/6/10

(B)

Lesson Unit/Topic

• PCR purification

Flow Chart

12/8/10

(D; Immaculate Conception Liturgy)

Lesson Unit/Topic

• PCR purification

.V Map

12/10/10

(F; Stocking Paraliturgy)

Lesson Unit/Topic

• PCR purification

.V Map

12/14/10

(B; Advisee Meeting)

Lesson Unit/Topic

• PCR purification

.study for test

12/16/10

(D)

Lesson Unit/Topic

• Test: PCR purification

• Introduce Quantitation

.Merry Christmas!

Lesson Unit/Topic

• Dilutions

• Go over test

Lesson Unit/Topic

• Dilutions

.

Lesson Unit/Topic

• Exam review

Prepare for Exam