Description: This course is designed to
introduce students to the theory and techniques involved in modern biomedical
science as applied to the discovery and analysis of human genes. The course emphasizes hands-on laboratory
experience involving basic molecular biology techniques in a problem-solving
format.
Text: Binder prepared by instructor for this class.
Objectives:
Develop
a pedigree and phenotype/genotype analysis which will comprise a
medical/genetic
diagnosis of a genetic illness as a working hypothesis.
Review
the Central Dogma and compare to in vitro DNA synthesis /PCR
Master
the skill of micropipetting for running reactions and
gel electrophoresis
Research
and analyze the differences between the various forms of genetic diseases
Integrate
DNA sequencing techniques into the final laboratory activities
Requirements: Students will need a
graphing lab notebook to collect data.
Lab reports and worksheets are to be kept in a folder. All lab reports will be completed in Vee Mapping (or alternative) format complete with concept
map and flow chart, typed on the computer.
Attendance: If a student is absent from
class and misses a test, she must be prepared to take the test on the
day she returns. Extended illness
make-up work is scheduled with the instructor. Late homework will lose one
letter grade per day. Students who are absent from class are expected to email
the instructor if the work is not listed on the website.
Grading
policy: tests: 40% quizzes: 20% HW:
10% Labs: 30%
Course
Outline:
Laboratory Safety Regulations
Recording data: Lab Notebook
Use of Equipment: micropipettes, gel boxes, PCR
machine, centrifuge,
photodocumentation
system
Solutions, Autoclaving, tip preparation
Principles of Micropipetting
Principles of Gel Electrophoresis
Introduction
to Class Problem
Use of NCBI Website: OMIM
Analysis of Inheritance Pattern
Construction of Human Pedigree: Diagnosis of the
Genetic Disease and
Hypothesis
Formation
DNA,
and Replication and the Central Dogma
Use of the NCBI Website: Nucleotide
searches
Introduction to the Genome
DNA
Synthesis in vitro: PCR
Amplification of DNA by PCR
DNA Analysis: Agarose Gel
Electrophoresis
RNA
and Transcription
Proteins
and Translation
Genomic
vs. Plasmid DNA
Restriction Enzymes
Recombinant DNA: Paper Plasmid Activity
Use of Webcutter and Nebcutter
Restriction Enzyme Digest Application for Class
Problem
Pedigree analysis/correction/reconstruction
DNA
Sequencing and Analysis
Sanger dideoxy
sequencing
Use of the Automated Sequencer for Cycle Sequencing
Use of the NCBI Website: BLAST
analysis
Microarray technology
Case study
Workheets
Analysis
Lab